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Promega pgem-t easy -ta cloning kit

Pgem T Easy Ta Cloning Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem+easy+cloning+kit/pgem+t+easy/pmc11575172-55-0-9
Average 90 stars, based on 1 article reviews
pgem-t easy -ta cloning kit - by Bioz Stars, 2026-10
90/100 stars

Images

1) Product Images from "C19MC drives nucleolar invasion of mitochondria and meiotic nuclear division in human cancers"

Article Title: C19MC drives nucleolar invasion of mitochondria and meiotic nuclear division in human cancers

Journal: iScience

doi: 10.1016/j.isci.2024.111132


Figure Legend Snippet:

Techniques Used: Immunofluorescence, Western Blot, Sequencing, Clone Assay, Cloning, Transfection, Subcloning, Recombinant, Labeling, Modification, Control, DNA Ligation, Plasmid Preparation, Purification, TA Cloning, Reverse Transcription, cDNA Synthesis, Knock-Out, Generated, Software

Related Articles

Polymerase Chain Reaction:

Article Title: Inter-specific interactions between carbon-limited soil bacteria affect behavior and gene expression.
Article Snippet: Recent publications indicate that inter-specific interactions between soil bacteria may strongly affect the behavior of the strains involved, e.g., by increased production of antibiotics or extracellular enzymes.. This may point at an enhanced competitive ability due to inter-specific triggering of gene expression.. However, it is not known if such inter-specific interactions also occur during competition for carbon which is the normal situation in soil.

Article Title: Genome survey and development of 18 microsatellite markers to assess genetic diversity in Spondias tuberosa Arruda Câmara (Anacardiaceae) and cross-amplification in congeneric species.
Article Snippet: Spondias tuberosa Arr.. Cam belongs to the Anacardiaceae family, an economically important family of plants whose fruits are consumed by humans and animals.. The aim of this study was to develop microsatellite markers using sequences from high-throughput sequencing and a magnetic bead enrichment method.

Purification:

Article Title: Inter-specific interactions between carbon-limited soil bacteria affect behavior and gene expression.
Article Snippet: Recent publications indicate that inter-specific interactions between soil bacteria may strongly affect the behavior of the strains involved, e.g., by increased production of antibiotics or extracellular enzymes.. This may point at an enhanced competitive ability due to inter-specific triggering of gene expression.. However, it is not known if such inter-specific interactions also occur during competition for carbon which is the normal situation in soil.

Article Title: Rhinomonas nottbecki n. sp. (cryptomonadales) and molecular phylogeny of the family Pyrenomonadaceae.
Article Snippet: The cryptomonad Rhinomonas nottbecki n. sp., isolated from the Baltic Sea, is described from live and fixed cells studied by light, scanning, and transmission electron microscopy together with sequences of the partial nucleusand nucleomorph-encoded 18S rRNA genes as well as the nucleus-encoded ITS1, 5.8S, ITS2, and the 5’-end of the 28S rRNA gene regions.. The sequence analyses include comparison with 43 strains from the family Pyrenomonadaceae.. Rhinomonas nottbecki cells are dorsoventrally flattened, obloid in shape; 10.0–17.2 μm long, 5.5–8.1 μm thick, and 4.4–8.8 μm wide.

Clone Assay:

Article Title: Inter-specific interactions between carbon-limited soil bacteria affect behavior and gene expression.
Article Snippet: Recent publications indicate that inter-specific interactions between soil bacteria may strongly affect the behavior of the strains involved, e.g., by increased production of antibiotics or extracellular enzymes.. This may point at an enhanced competitive ability due to inter-specific triggering of gene expression.. However, it is not known if such inter-specific interactions also occur during competition for carbon which is the normal situation in soil.

Article Title: Dynamics of the SAR11 bacterioplankton lineage in relation to environmental conditions in the oligotrophic North Pacific subtropical gyre.
Article Snippet: A quantitative PCR assay for the SAR11 clade of marine Alphaproteobacteria was applied to nucleic acids extracted from monthly depth profiles sampled over a 3-year period (2004–2007) at the open-ocean Station ALOHA (A Long-term Oligotrophic Habitat Assessment; 22°45 N, 158°00 W) in the oligotrophic North Pacific Ocean.. This analysis revealed a high contribution (averaging 36% of 16S rRNA gene copies) of SAR11 to the total detected 16S rRNA gene copies over depths ranging from the surface layer to 4000 m, and revealed consistent spatial and temporal variation in the relative abundance of SAR11 16S rRNA gene copies.. On average, a higher proportion of SAR11 rRNA gene copies were detected in the photic zone (< 175 m depth; mean = 38%) compared with aphotic (> 175 m depth; mean = 30%), and in the winter months compared with the summer (mean = 44% versus 33%, integrated over 175 m depth).

Article Title: Genome survey and development of 18 microsatellite markers to assess genetic diversity in Spondias tuberosa Arruda Câmara (Anacardiaceae) and cross-amplification in congeneric species.
Article Snippet: Spondias tuberosa Arr.. Cam belongs to the Anacardiaceae family, an economically important family of plants whose fruits are consumed by humans and animals.. The aim of this study was to develop microsatellite markers using sequences from high-throughput sequencing and a magnetic bead enrichment method.

Article Title: Rhinomonas nottbecki n. sp. (cryptomonadales) and molecular phylogeny of the family Pyrenomonadaceae.
Article Snippet: The cryptomonad Rhinomonas nottbecki n. sp., isolated from the Baltic Sea, is described from live and fixed cells studied by light, scanning, and transmission electron microscopy together with sequences of the partial nucleusand nucleomorph-encoded 18S rRNA genes as well as the nucleus-encoded ITS1, 5.8S, ITS2, and the 5’-end of the 28S rRNA gene regions.. The sequence analyses include comparison with 43 strains from the family Pyrenomonadaceae.. Rhinomonas nottbecki cells are dorsoventrally flattened, obloid in shape; 10.0–17.2 μm long, 5.5–8.1 μm thick, and 4.4–8.8 μm wide.

Transformation Assay:

Article Title: Inter-specific interactions between carbon-limited soil bacteria affect behavior and gene expression.
Article Snippet: Recent publications indicate that inter-specific interactions between soil bacteria may strongly affect the behavior of the strains involved, e.g., by increased production of antibiotics or extracellular enzymes.. This may point at an enhanced competitive ability due to inter-specific triggering of gene expression.. However, it is not known if such inter-specific interactions also occur during competition for carbon which is the normal situation in soil.

Cloning:

Article Title: Inter-specific interactions between carbon-limited soil bacteria affect behavior and gene expression.
Article Snippet: Recent publications indicate that inter-specific interactions between soil bacteria may strongly affect the behavior of the strains involved, e.g., by increased production of antibiotics or extracellular enzymes.. This may point at an enhanced competitive ability due to inter-specific triggering of gene expression.. However, it is not known if such inter-specific interactions also occur during competition for carbon which is the normal situation in soil.

Article Title: Dynamics of the SAR11 bacterioplankton lineage in relation to environmental conditions in the oligotrophic North Pacific subtropical gyre.
Article Snippet: A quantitative PCR assay for the SAR11 clade of marine Alphaproteobacteria was applied to nucleic acids extracted from monthly depth profiles sampled over a 3-year period (2004–2007) at the open-ocean Station ALOHA (A Long-term Oligotrophic Habitat Assessment; 22°45 N, 158°00 W) in the oligotrophic North Pacific Ocean.. This analysis revealed a high contribution (averaging 36% of 16S rRNA gene copies) of SAR11 to the total detected 16S rRNA gene copies over depths ranging from the surface layer to 4000 m, and revealed consistent spatial and temporal variation in the relative abundance of SAR11 16S rRNA gene copies.. On average, a higher proportion of SAR11 rRNA gene copies were detected in the photic zone (< 175 m depth; mean = 38%) compared with aphotic (> 175 m depth; mean = 30%), and in the winter months compared with the summer (mean = 44% versus 33%, integrated over 175 m depth).

Article Title: Development, characterization and use of genomic SSR markers for assessment of genetic diversity in some Saudi date palm (Phoenix dactylifera L.) cultivars
Article Snippet: .. Cleaned elutions of the SSR motifs were used for TA cloning using pGEM Easy cloning kit (Promega, Madison, WI.USA) flowing manufacture's recommended protocol. ..

Article Title: Genome survey and development of 18 microsatellite markers to assess genetic diversity in Spondias tuberosa Arruda Câmara (Anacardiaceae) and cross-amplification in congeneric species.
Article Snippet: Spondias tuberosa Arr.. Cam belongs to the Anacardiaceae family, an economically important family of plants whose fruits are consumed by humans and animals.. The aim of this study was to develop microsatellite markers using sequences from high-throughput sequencing and a magnetic bead enrichment method.

Article Title: Rhinomonas nottbecki n. sp. (cryptomonadales) and molecular phylogeny of the family Pyrenomonadaceae.
Article Snippet: The cryptomonad Rhinomonas nottbecki n. sp., isolated from the Baltic Sea, is described from live and fixed cells studied by light, scanning, and transmission electron microscopy together with sequences of the partial nucleusand nucleomorph-encoded 18S rRNA genes as well as the nucleus-encoded ITS1, 5.8S, ITS2, and the 5’-end of the 28S rRNA gene regions.. The sequence analyses include comparison with 43 strains from the family Pyrenomonadaceae.. Rhinomonas nottbecki cells are dorsoventrally flattened, obloid in shape; 10.0–17.2 μm long, 5.5–8.1 μm thick, and 4.4–8.8 μm wide.

TA Cloning:

Article Title: Development, characterization and use of genomic SSR markers for assessment of genetic diversity in some Saudi date palm (Phoenix dactylifera L.) cultivars
Article Snippet: .. Cleaned elutions of the SSR motifs were used for TA cloning using pGEM Easy cloning kit (Promega, Madison, WI.USA) flowing manufacture's recommended protocol. ..

Article Title: Genome survey and development of 18 microsatellite markers to assess genetic diversity in Spondias tuberosa Arruda Câmara (Anacardiaceae) and cross-amplification in congeneric species.
Article Snippet: Spondias tuberosa Arr.. Cam belongs to the Anacardiaceae family, an economically important family of plants whose fruits are consumed by humans and animals.. The aim of this study was to develop microsatellite markers using sequences from high-throughput sequencing and a magnetic bead enrichment method.



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Image Search Results


Journal: iScience

Article Title: C19MC drives nucleolar invasion of mitochondria and meiotic nuclear division in human cancers

doi: 10.1016/j.isci.2024.111132

Figure Lengend Snippet:

Article Snippet: pGEM-T Easy -TA cloning kit [Usage: Manufacturer’s recommendation] , Promega , RRID: NA; Cat# A1360.

Techniques: Immunofluorescence, Western Blot, Sequencing, Clone Assay, Cloning, Transfection, Subcloning, Recombinant, Labeling, Modification, Control, DNA Ligation, Plasmid Preparation, Purification, TA Cloning, Reverse Transcription, cDNA Synthesis, Knock-Out, Generated, Software